{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "95706658-53e3-4ab2-934c-6a07e861ef4e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 35304, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "593e946d-d334-4a52-9c7c-9aef3c618cd3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 35816, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "0cdc70d3-47d3-4334-b787-046c81f498e3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 36324, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "3cce785f-6765-4081-afeb-fac0b3c35399", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 36834, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "f0362f0b-3dda-4d27-a920-5bbf3c0c5e8b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 37347, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "7ead1ef7-79e6-4aec-a0de-11b6d4a26a18", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 37857, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "cb41f975-c93a-491d-a018-e1f8ec9fe4ad", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 38368, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "0ca335f0-ed19-4f50-9d09-493b8817d62d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 38882, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "8e0aed81-3357-4634-85f6-e86d7a491632", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 39392, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "7034388d-2ad2-44c2-a8e1-c48686f69ebe", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 39909, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5ab74814-996e-41aa-87da-97e2d373eac4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 40419, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "fe252163-c45b-4e67-bcc0-ded9e8a319e8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 40926, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "7c3e670b-78fc-4046-8b53-0209bd641e7a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 41439, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "9f5583e8-4d3d-4a72-b95d-ddde6321a2ae", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 41950, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "68062a9c-c2d6-4032-af0f-a8b0b04ba423", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 42462, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "42d83fa8-879b-40de-9e70-f2f9358296d5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 42974, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "09e00556-d284-4126-9af8-e64abfa22b32", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 43490, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "55fc6a5f-a4bf-465f-80e0-6014b326f0e2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 44701, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e5cf1434-66a8-46d3-9a8e-c08c9b7c0eb2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 45012, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4f80f469-299c-4bd8-a97a-b68f0dfc31db", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 45315, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "34a28fd5-767e-4f33-bb8e-45280820fed8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 45623, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b895b5a6-d579-44d2-b1d2-21b06b84f45d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 45942, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f69d31a4-96fb-4a53-8110-43dbed8416e0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 46257, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4c3946b9-8d7f-4679-915d-182d65ed03fd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 46561, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6bdce31b-9bf0-485a-990e-7c698a06b99e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 46866, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9275dd47-b556-4590-9272-dd62f5e53414", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 47169, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8a82bd2e-5b46-4750-8e8f-9be9186b1bda", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 47475, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4683727f-4fc8-4e03-aab3-2b06fd004c25", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 47794, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e372d1f3-4845-43e3-8a63-be5c5252ee41", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 48098, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6745ba1f-2b9e-4a07-a39c-4e23aca8526a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 48432, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "27f848b0-5928-4fdc-974f-018d938ce312", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 48737, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7ab9fbea-95cc-46e7-acfb-e25890bcd1c0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 49040, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "dbc65a53-d718-4fcd-b73f-533fec90fc24", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 49343, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2541bcc4-24a9-47be-96c5-e32123535ec4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 49652, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ebe4a8e7-1f36-49c5-8a8d-721af9036a15", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 50352, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6045bbd7-065b-4440-a67d-9e20aaf8c5b6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 50728, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1b67013f-05b1-4559-ab10-99c972ca9da4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 51088, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "392b9444-c5bd-4aa1-9104-a07e26803134", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 51444, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "70c4d046-58b5-45a2-992f-5fb4f755edaf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 51810, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b32a60f1-d580-4da6-8141-bc7a28c64e76", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 52174, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "99baa0ff-a87a-4383-8238-5f759b6f37ac", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 52546, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b3eb1232-3106-464b-9adf-9428aeb1ace4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 52911, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b0549cc5-1482-487b-9771-047de1e2e7e3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 53279, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b61deea6-8a0b-429c-aa3c-3e831de624a9", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 53645, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4ddeb3fa-7a79-476f-976b-e62c1d897711", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 53999, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "533e396e-944e-4018-b401-e0eeca18bfce", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 54364, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6cac5238-790b-43d9-947c-e0233826063c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 54717, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f7790986-f667-42b0-b16d-b4e2fb503db5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 55089, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:02:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1e2b078f-75cf-47f4-8b64-62df4b7af64b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 55453, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ee1ffd67-c7fa-4ca2-a827-99910de64076", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 55804, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b4b019ee-b4f3-4cef-8f3e-5c96438c10df", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 56161, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "16ca4c71-3702-4fe6-9e51-dc5c19fe9cd0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 56953, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7f7a0be5-cc15-4e54-8b90-c989cd428263", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 57425, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "98d441e6-e580-48b0-9286-cec9356e6335", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 57886, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "19db798f-8079-4655-b571-8e07f30c85cf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 58349, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "dd3376f1-bac7-43c0-9dc2-1007f4841afc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 58814, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ca7f1f96-7f17-423f-b6ae-4137ae9f8a60", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 59277, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ee228043-9324-4936-a0d6-3b7e82877cd6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 59740, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1a7657b7-de8a-44c7-b992-f261f85f2961", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 60209, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "be1e104c-dc53-4b56-8196-13a7521c0c29", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 60672, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6a1c4ee6-6931-4b3d-a786-c002cd1cc189", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 61132, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ce32fa67-30c8-4751-95e9-7581a82391e4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 61596, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "027d78eb-0ab4-41ad-8259-aa741217d0e3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 62061, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0c8ae10c-23b2-4f9e-9b09-959a8d24e118", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 62523, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "90861548-9c22-4fb9-9bd6-08010e1f18b4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 62987, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "81d9be35-4e20-4516-bb0e-057a73d80c0b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 63450, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f1354c12-4a64-4ccc-a664-5c4759171674", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 63916, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6e95df49-52de-4db6-85c4-447beb6bd10e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 1, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, -398.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, -398.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75322.1, 162.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18559.4], "PIEZO": [170.1], "XYStage": [ -75883.1, 162.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq10_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -6315.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -6315.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -74678.5, -5754.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq10_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [18511], "PIEZO": [170.02], "XYStage": [ -75239.5, -5754.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq10_Thalamus_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "08ad24bd-33b4-4b04-8085-703767cdff65", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-19 01:02:40 -0400", "Date": "2019-06-19", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 1, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq10_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 8, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "100003000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 64381, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq10_Thalamus_02", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "18477.4000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-15.4700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "2", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "137.2200", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -75322.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.1, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "1", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-19 01:03:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "1", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -398.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }