{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b191cb91-f5ff-4ad3-b4d9-a735d54a9e73", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 354879, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "dd807e8c-393d-4241-baed-35c5bb56814a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 355401, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "7abe5c2b-bc8b-40e4-88be-16afdbbc47b3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 355911, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "35cec196-d03e-46c2-ab7e-62673802688e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 356426, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "a189d9cf-d371-46d6-b91e-6d6401ea2ed3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 356935, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "12ea9f59-a47b-4fd6-967d-6cef55b557da", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 357446, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b877e76c-f397-4cb4-99c8-d8fd9ae80062", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 357963, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "224ded76-838d-4617-8269-fee724114caa", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 358469, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "f4d0783b-87a8-47d5-a217-5d20ce84d080", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 358983, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "97a72218-5979-42cc-933b-f995961b22e8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 359493, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "15c7efd7-fe3c-4adb-830e-97552dc4a03f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 360014, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "e0847a66-e720-4a0f-9978-46df3e41f905", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 360532, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "d43110b6-91d1-401a-a921-629e8bcf797e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 361045, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "a4211e6e-8fea-4cbc-a0c4-83dc55403dd4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 361560, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "bbc3fbab-fbfe-41b3-bc24-2ae2c30666df", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 362066, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "79429013-8f0c-4d69-9da5-e10d487fde7b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 362577, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ea468415-2b0b-49f9-8177-116b832a0597", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 363093, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bca9445a-beb9-4b57-afaa-b72ec01db66b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 364291, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cbdf0f1e-f33e-40ab-9410-49bde584859e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 364600, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b9255ffa-193a-4ea0-887f-802de347b7f4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 364902, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "10f37547-2227-492e-bf50-d6d14e502f72", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 365207, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e2ac5ac6-2d5f-4dc0-8a96-05d73f5abfcd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 365528, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "89205ed8-7d45-41a2-b4c3-10c97e7f59ca", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 365840, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "03e9f286-5595-4a6e-980d-49fbb1897e98", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 366149, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4f355d98-9ef2-4b05-8c59-0cb9c37e5177", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 366454, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "96adfdbd-c45c-43d1-996f-f59f9fbdde25", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 366768, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5340bb19-91a3-48b0-a690-49baad7b69b0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 367115, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fdad67e4-4ffb-4244-82a5-78ce7a5e6734", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 367450, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "da6048de-0520-4eb1-b853-c8c83d1a319c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 367768, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0517b1a4-418d-49c7-a14b-d300fb67ed34", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 368086, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7ce6b4fb-445f-4267-b258-0fe75a39c012", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 368388, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f589cfad-573b-41bf-a057-9f7792dce5d6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 368693, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d6622b1a-0fea-40ec-9747-b66fc96554b5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 368996, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cdbaf5b5-507a-4d8f-a301-9b20f609f14a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 369305, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "08242570-cea9-475f-947e-4418a81883c7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 370000, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "53dc3b22-d891-4aa9-8db5-bf1af2ad765f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 370381, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9a014208-a081-4044-a3db-884e50f20c6e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 370745, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3dd9e4e9-a65b-4403-b8fd-5af860e5cfb0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 371114, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "eddc488c-8856-47ae-9e81-5a392d215601", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 371479, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "baca5cac-ad45-44b2-99b7-45b089f269ec", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 371847, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0feccc3f-9b08-4550-baec-faad7508d48b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 372216, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "84d4f31c-f44b-41d4-ac2b-53142aacce52", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 372579, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b7a51bb5-2106-4c80-8664-1d72a0385567", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 372946, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "649a1642-7e13-4705-9cef-78c00870824c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 373313, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f8cd3291-f278-40ac-8f16-62fef80fe848", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 373665, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "58f29fe4-c213-486a-a7d7-d7a36917be76", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 374018, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f6e6f349-16d5-4de0-96f7-02a541c35123", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 374385, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "86adc950-d8b4-4f67-bd48-1d4563a0e04e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 374751, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b9e4b0e7-78ed-43b8-aa5a-cb9dcdd91989", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 375125, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8fc69222-25af-495e-8499-22b9530e03a2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 375488, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9ac37b26-b434-4b7e-b37d-ee14cdee898a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 375861, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "096f5d7f-75d7-456d-8e9a-762ef5a5193f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 376631, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5a3d3e81-aa3c-40e1-9c66-2df1fa345a06", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 377094, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4951c304-7a92-42a0-9667-0ac6f051d9ae", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 377560, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "11c42447-50ae-40b1-8625-117a0610489c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 378034, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "850e85b1-d2d6-4516-aae0-3567483a7a45", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 378500, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "89271424-105d-45b4-95d7-125b67616383", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 378962, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "071aec07-7487-4cd4-82b0-30c1ffbaa101", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 379424, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "311257ad-18f6-4188-8747-56370eb6069b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 379888, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "850cd35e-7fd8-4d62-94f8-198e7ac394a0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 380355, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8e81075d-977e-4b43-b808-69a5d81e3f44", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 380816, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "032fea1a-bb89-4f72-8619-faeea30e3e5f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 381278, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5ca29c14-834d-42e5-9636-32d5e3abb6e1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 381742, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e88c0298-4501-4d32-94a5-5e1ea640e86b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 382205, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fb1d2341-67e1-4d09-9d16-ee1dcaae2c18", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 382670, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7d82f327-0e99-422e-806a-74633d6a2449", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 383135, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f6125859-e612-48ec-938c-38704afd413b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 383595, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b3af2a29-468f-4fbb-aac4-874a7535cac1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 11, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:04:29 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 11, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 384067, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -41078, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:04:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4250.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }