{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "8a7c22ed-09ca-42ec-a61a-f1f2dd2a45f6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 757419, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ef2e8dd2-9f52-4692-827f-5390406adcba", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 757923, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "9348793a-a091-4c7d-b87b-51f33090b72f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 758439, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "48cc9af9-f607-48b9-a35f-475978bd64d0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 758945, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "06a93c0f-945e-4072-8d47-ba290f458154", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 759483, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "bb0dcb69-8e8c-4a97-902d-5ea4eb0e331b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 760001, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "de5485c5-357c-46c2-a77f-693d0deeb473", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 760514, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "388b6770-61c6-4865-be1f-04a36381cd5c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 761024, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5580f815-1489-4639-98ba-d5babd02a268", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 761541, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "e2faee3f-7fc8-4eb1-9edc-bdee2e60bcfc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 762047, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "2629d723-19ab-47ae-8413-0c2c32403f9a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 762558, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "66fc6e21-803d-4894-9105-a38d60819a15", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 763077, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "cccb5cdb-3d9e-43ff-9e7d-f63ffa9cfd0b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 763600, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "1e8fdf7e-f6bc-4b66-9a3f-466702d3edd0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 764109, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "4605a7e1-e64c-44f3-8500-ac48003318e8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 764621, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "8db169c8-8b95-49f6-8b5d-1377883e2fe3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 765133, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c4bf8aca-a3ca-47ce-bf83-eb52c5363bac", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 765648, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0cecb229-bcc5-48e0-8674-86a9e89891a3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 766848, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "01e3084f-7084-4212-b42c-fe1c4a30449e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 767159, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "eccab9ea-39da-4237-80c4-37eea7714b9c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 767476, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fc2fd02e-1af0-4e73-92e2-c41718e8dbc9", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 767793, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "107e1cf8-fc27-4a8c-a2a1-afe9a0d87bdd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 768114, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8d71232c-c6fd-4abd-a778-3dc1e98ee620", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 768434, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cae8ebf4-4338-456b-ad2a-513f04039dfe", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 768754, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c7fd0f5f-f01d-4bf7-8b49-150c8f6fb271", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 769073, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8824a1da-fe1c-4ba6-af41-fb18a6c82c5c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 769393, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "17961245-6cac-41b2-b263-50935fb31686", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 769697, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c3df1a96-4394-42ee-b485-a6598b575ea8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 770000, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "feb244f7-b018-460e-802b-b659b2e5439e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 770304, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "779e686e-e326-4954-97c8-5ef09490f2a6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 770622, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "180c798f-ac14-4f91-b266-643431030370", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 770927, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "03acc5ae-0757-47d0-8637-d7b72b2e954f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 771230, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bd80bf22-3f18-4aa2-8ae4-09a3d2c67ccf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 771533, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "66554f15-f1a7-4817-8ac9-5081c12fd588", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 771846, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "adb6135d-edc1-48a0-b52f-fdb9a0c3fa2e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 772566, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e910cbc2-2aca-4f21-8e13-08918ff5a110", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 772931, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "223decf2-9f24-4333-b91d-f842c67dc149", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 773300, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "992f7beb-0bd0-47bf-82e5-3f2c7257ee3e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 773661, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f282754a-760b-4e24-a273-225a46ce5dcc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 774014, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d3ec2ac7-fb5f-4e9c-8df5-281cabe3e931", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 774381, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "df8fe342-ef4c-435e-a912-eb4cd03a13ad", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 774748, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e302dfc5-bbac-49e7-b32d-a27a42a9dc4c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 775117, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d496426b-de12-4c2c-9403-3a4dd0e5e96a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 775484, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e0183a64-21a4-40e2-9a6d-4840110d3d3b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 775852, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2625e41f-df5e-4e2c-9374-020db4516535", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 776225, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "768a6757-27b5-4da5-bbd6-4c33047ab2ce", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 776588, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "68dc6c87-aa57-4acf-99b7-d09b813a350c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 776961, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4d272d5e-652a-4efe-8bb1-8ee05d1f27e6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 777323, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e564979f-b76b-446b-8fe7-6bfce31ba9b2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 777690, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "503afd1b-4031-4b86-a77d-4ec2fd826fd6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 778062, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1f69ac15-1816-4261-a4bc-44b081375655", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 778426, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "063243b7-bcc4-41dd-88f7-098301ea98ba", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 779202, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "85ac49f1-6e00-4a3a-9e0d-13a3ed8ac45d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 779660, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "01f70f4b-1356-4c52-8de8-535d0f63dc1b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 780127, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c8746ca6-cc9e-4960-8456-428f6c0e9ce1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 780601, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8d9f1300-4973-4eab-8a56-004b8085b2f2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 781069, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f7ac8040-1a3a-43dd-be76-ac967f1b1017", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 781533, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f86483ab-5c12-4a23-af30-70585e002591", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 781996, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "298d1330-9aa9-47cd-a2a5-02eaeb5e2f28", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 782457, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9f8201fb-b3b9-48e5-bf8e-a065c5a91b84", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 782923, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3d7f8a07-829a-4847-934c-1f344c1e81b8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 783385, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4eded7b0-3373-4f20-b9e0-f445187b92dc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 783853, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "43cb0a36-345a-47fb-974c-524041b83b75", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 784312, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "93fb6ead-b923-4fc7-be83-5deb22843c25", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 784775, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c9292633-ef8a-4ef2-8bc4-793af05c8abf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 785238, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "86d0ed42-3261-432e-b6e6-1380c2709201", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 785703, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0952016b-b85f-4a6e-aedc-7719708a6485", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 786169, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8c28cc10-cc48-409f-8861-e0ee16b13998", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 24, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:11:11 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 24, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 786635, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34123.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }