{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "46e59ac9-281d-4351-8221-e119ce42b143", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 726408, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "bf54b4dd-8f35-4a7e-827e-118585d3cf35", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 726911, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "cb8e89ec-272e-43cf-9807-2ee9e9f3cce6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 727433, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "c99a0d49-4d9a-491c-b4e9-195e9eb3b309", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 727954, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "1587b262-0630-4aba-b33f-94f23182910f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 728462, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "bb963e29-5da1-4a73-8df8-4bae88d46e65", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 728974, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "7b148328-ca29-49bb-b19e-373cc9900083", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 729485, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "fef2beb8-2f67-4961-9260-dff5b8c6462f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 730001, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "41617a71-ecfe-43d4-8bb5-533036144014", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 730508, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "8ca2258a-4c61-4ef6-8600-3f26fcc81b49", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 731019, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "93051d1e-6d6a-4f0d-9add-44ecdf9f616f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 731531, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ae2c5409-d6cd-4079-9a61-808bb773e50c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 732181, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "436c9b92-83b4-4b8d-8850-95f0caa46e3e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 732700, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "18e1b6f3-1111-4b18-b5a5-7589711b5f14", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 733215, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "f9710e1d-d1e8-4c64-b218-4e51cfab7a86", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 733746, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "f2c19d72-4251-4c0f-9d2a-3480c4b2b27f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 734265, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d729dfd8-0f39-4f12-a6c0-44854cf177a7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 734779, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7b56f169-4609-4a8d-a727-8c97966bae1b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 735992, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5377e624-0467-4a70-a1a2-11f8f7d617b1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 736309, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f90bd762-6add-4f0d-aece-672fa3717180", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 736621, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "315ca93e-85b3-472e-a2e9-2efc77852b02", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 736929, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b6b9e17c-21ec-4b7f-a434-86ed13a1ca5e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 737249, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0b86b1fb-5d71-4655-8059-2ea6d28ab501", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 737577, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "466e450f-6f4d-4962-90a6-3287094d2b21", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 737904, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3815ff97-3ad9-4c84-a2d3-8a2a8c2e9073", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 738223, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6cc883ec-fb38-484e-88cc-2344445f8aa7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 738541, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f5023605-98d2-4575-83d9-6fac183fd47d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 738849, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a0382850-0ca5-46d5-a91a-3c90da8f0a90", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 739164, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "eef22daf-2695-4574-80c3-ab75092b592b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 739470, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "036428e6-fcc3-42b0-a2a6-2e9d42d2ef72", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 739788, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e4b115ae-ca67-4b1f-9e0b-300b71c00410", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 740111, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "03364c83-12c3-4cbb-9fe8-5f5a40643be7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 740428, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8982040c-d22d-441c-8458-e228d03a401b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 740748, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "95110563-bf72-4b77-bccb-26c756b47590", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 741091, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8222ae6e-0706-40ff-91b9-9c10847436f4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 741797, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7229bb5d-de58-481d-b547-8d5c7cc9d77d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 742160, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2060d333-4a42-4364-a8ba-c910b91fdbc0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 742526, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9ea3a363-507d-4f2c-9545-6a597b033d86", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 742890, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "df1d1e56-1f6e-4361-b047-a5afa30afc27", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 743260, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5a8ea6ad-d7b8-41bd-8e7c-16405d628080", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 743626, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a36013d0-c505-437d-8cfa-616e0439d715", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 743999, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "17565c57-f21b-451f-9dbc-85b396163919", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 744361, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "02d36db4-92ff-42ef-bc3a-15b3aa8029e8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 744728, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5b1d34a1-dd2a-46bc-8d64-8906a759353c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 745081, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0b56bcea-520e-4890-9c9a-e8bfe51589a6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 745447, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:10:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2edab544-e597-4557-a6e7-6b61a10305df", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 745815, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b540cc36-7d02-4559-9f6a-4f4d26a69384", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 746183, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ed2e4001-bc6f-4aba-8065-06682f26a0d2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 746557, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b0315bb5-5e2c-47a8-8762-5749b906780e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 746924, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e6702155-9dd6-45e7-899b-778df8709450", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 747290, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f89d88d5-0458-4b00-b825-301083b210d8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 747658, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "43ee57eb-ae93-4ec8-ac96-7e574cb10949", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 748451, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "60168613-c0fd-4746-b3f4-5bd1b4c19a97", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 748929, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a1e2030d-841f-4719-95e4-0fb2351b34fa", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 749384, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "89c0266f-d66e-4b4e-a503-e3e74bf282a1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 749849, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0ea84875-f329-44d8-972e-1133b3adfd43", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 750312, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b293df76-61a2-4cd3-92ea-4069ea57d2e5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 750776, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6b9ac254-7b7f-45ff-849c-f845ce5bdd88", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 751239, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5e769a30-3c61-4b1a-b64e-495a21704527", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 751702, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "37a3168d-3262-49df-bf61-faeb58d59390", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 752167, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c761e442-32cc-48db-8608-a92c7d30cda1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 752629, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3b313b9d-ec11-4cc3-ac48-cf83f202c669", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 753092, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fc16b0f7-557e-470f-9e72-d3506600accf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 753557, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0259a3ef-3009-4426-ae64-c3a724422001", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 754026, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c53a75af-9089-4872-9727-3a6c54bd2b5f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 754483, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "82f508ff-34df-436b-a5b4-2c26dc6738c4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 754948, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7a6a8633-f5cf-4bc6-9750-ac60e99396b2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 755416, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "718c9d89-ad73-4519-bae9-d31c9006e9dc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 23, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 4443.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 4443.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -38823.5, 5004.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.6], "PIEZO": [169.975], "XYStage": [ -39384.5, 5004.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3128.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3128.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3128.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 3689.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 3689.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 3689.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41639, 4250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -41078, 4250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19808.4], "PIEZO": [170.015], "XYStage": [ -40517, 4250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 2454.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 2454.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39230.7, 3015.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19782.6], "PIEZO": [170.1], "XYStage": [ -39791.7, 3015.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2314.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2314.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2314.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 2875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 2875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 2875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34684.9, 3436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -34123.9, 3436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19788.3], "PIEZO": [169.9], "XYStage": [ -33562.9, 3436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3569.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3569.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3569.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -3008.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -3008.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -3008.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -34100, -2447.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -33539, -2447.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19722.2], "PIEZO": [170.03], "XYStage": [ -32978, -2447.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -3456.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -3456.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -38569.7, -2895.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19700.4], "PIEZO": [169.995], "XYStage": [ -39130.7, -2895.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -3251.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -3251.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -3251.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2690.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2690.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2690.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -41119.2, -2129.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -40558.2, -2129.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19721.6], "PIEZO": [169.98], "XYStage": [ -39997.2, -2129.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1668.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1668.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -38468.6, -1107.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19755], "PIEZO": [172.55], "XYStage": [ -39029.6, -1107.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "cf4d79a2-6dc0-491e-83bb-15a8c593a148", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 20:10:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 23, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 52, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 755879, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq05_Contra_07", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -34684.9, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.9, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 20:11:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 3436.5, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }