{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "0f6c7bdc-ac1d-4a49-a1d7-d5f4ce9c1c12", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 799890, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "434f059e-5e8e-4a71-881b-3ab3458e9f6f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 800397, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "601952a5-32ff-4932-92e0-0a89363b2e35", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 800912, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ec34de50-8590-4a18-ba17-ee97a63f3500", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 801419, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "48569cb5-4b15-4c59-b16e-ce8467334646", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 801935, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "8f138e51-4b51-4e16-956e-688882a02502", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 802445, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "110d6f7b-dde8-4f48-b946-695590b67643", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 802954, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "0fbc8980-174c-47ea-b43a-3c0738002d2f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 803467, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5b003d95-9c23-4268-8b04-45d0ccd5cb6e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 803977, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "a2b7eb6a-7b31-4f84-a10a-d18e5517e8d3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 804490, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b2547bfd-097a-4a32-832c-8eea42adf3f8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 805000, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "39c56ecf-21da-4695-b7a2-bccb21098fc5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 805517, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b358928a-2a8f-4114-b34d-5b9342553f71", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 806071, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "f0a3e8e2-608e-4c0a-bfa0-fda525da7d2e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 806582, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "2119c951-0034-4246-baec-2bcbba959533", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 807100, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "082c887d-aaeb-4247-a962-3e3ac6bbde6a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 807613, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b6175653-230a-4791-9fdb-065c014014e4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 808123, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f4fd2296-f9fd-4d71-8299-1a5463b607c2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 809335, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cf8404f1-a320-4ecc-a423-96686a1ca3b4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 809650, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e7a39f14-ae5d-410c-8764-3d1bdfb2b524", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 809964, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1f1554e5-2941-4246-ba86-11c5d7c4081f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 810288, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3f8e017f-4477-4e59-b8f5-9beb4feb0a21", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 810608, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "42c81212-1859-4962-8b3c-e887ed706a99", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 810923, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d814cfc9-a5bc-4818-adfa-8259c6d41087", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 811226, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "34e4cfc9-97eb-423c-bfd3-401b9b8c91c5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 811529, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "be402727-ac33-4056-a9c4-3957ed52d928", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 811835, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "903ee9d4-1185-4166-a2b3-8364b138736c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 812154, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9b8904fa-bc5d-4b62-b380-c44ef816dd32", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 812462, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6cd72d1f-bfc7-4547-96a8-cc4717bd0203", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 812777, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cfdf2f0f-8546-48da-963f-ff31af602f2d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 813079, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d233f06c-7cea-47b5-bbe6-00c4a24ee2e9", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 813405, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "78723bcc-8150-4282-9962-7d005121069e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 813720, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "26361151-eeba-4dc7-bb67-31c101bde279", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 814024, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a3a07311-f2da-4126-a1ca-421d2c1d0626", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 814337, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1d57bdb2-4d80-4798-9dff-7b764028b212", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 815056, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "be1b9331-3b17-4662-8c62-6fe612d85a2c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 815471, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6edee27c-f0fb-463d-91c3-a12c8868c982", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 815833, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f375c4ca-5fbd-4ade-9b16-2f95c4480ca4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 816199, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6b39ee72-b92a-42bf-8d18-b940c1bb9141", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 816569, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3078c520-6a89-42e5-bea8-34ade81a7ea8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 816939, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "09301322-af7e-426c-803a-a87e382c76df", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 817308, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6981c038-6963-4161-b105-bc1a77bd83fc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 817671, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3fc1dc80-532b-4561-98ae-60cbd3d92940", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 818039, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0fd089dc-40dc-455e-a425-da0fd6162bd2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 818405, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3f164587-b727-4777-ac73-cd68c39d0134", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 818775, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "83ca4a0e-482d-490c-bae5-15d22cdc929e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 819142, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a1b94a22-c354-4dca-a27f-bdc71b86a238", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 819508, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:08:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b70d30ff-61aa-4a1a-bc6e-6b52f31819d8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 819877, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9c7784cb-1084-4a98-b484-8761ee70cb52", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 820243, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "442ab6dd-fd28-458e-8268-bde10e9e6736", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 820597, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4702c1eb-0be0-47b7-abcd-6f5ce459e7ee", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 820965, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fcdc8ae4-2ce7-4ceb-9e11-abb66da6aa27", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 821741, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d2a6644c-3c02-421a-a243-0d4d071554d8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 822198, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7a94925d-91ec-412d-9bc6-d2feec47b2c0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 822662, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1ec77928-a3df-475d-9a06-7d8bd131e4f4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 823125, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e4fb7c39-ddc4-43dd-a18f-e666fe17880e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 823590, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ba98ca90-37a0-4265-b451-fc7513aed3bd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 824055, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "529d1f79-6720-4147-8ee2-6574924ad1c4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 824515, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5967fa96-7899-4c54-95ee-10738750cde2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 824980, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2584d1b9-2aa3-4fb2-970a-66c98135da3c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 825446, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a72b8f5e-fe4b-4ea4-8189-8ff0ad06c8a9", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 825908, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a4de13e0-e288-4c3e-b202-be560289a072", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 826371, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0c8bf3e3-0316-4394-93ed-d66b8ba7cc8b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 826834, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8bc3097b-2920-4fe7-adcf-737de87f3cc2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 827297, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ef83ad2a-a2cb-46e7-bba6-e320e8da180e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 827760, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "dab72311-c0e3-4d5e-b95b-5995756c7e2d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 828225, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "be1168e6-f071-4b74-8d7b-9cf37c9b51c5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 828711, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "579c906b-b359-4640-8444-a54722e1cad5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 25, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4100 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4100 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 38205.2, 4661 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19369.9], "PIEZO": [170.1], "XYStage": [ 37644.2, 4661 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2298.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2298.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2298.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 2859.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 2859.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 2859.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35268.1, 3420.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 35829.1, 3420.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19361.4], "PIEZO": [170.04], "XYStage": [ 36390.1, 3420.3 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 1883 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 1883 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37971.2, 2444 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19359.7], "PIEZO": [170.025], "XYStage": [ 37410.2, 2444 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 2952.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 2952.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 2952.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 3513.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 3513.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 3513.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 42689.2, 4074.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43250.2, 4074.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19377.7], "PIEZO": [170.06], "XYStage": [ 43811.2, 4074.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -5162.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -5162.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -5162.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4601.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4601.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4601.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43023.2, -4040.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 43584.2, -4040.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19334.3], "PIEZO": [170.045], "XYStage": [ 44145.2, -4040.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -5411.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -5411.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 38118.7, -4850.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19306.3], "PIEZO": [169.955], "XYStage": [ 37557.7, -4850.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4909.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4909.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4909.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -4348.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -4348.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -4348.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 35461.7, -3787.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36022.7, -3787.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19322.1], "PIEZO": [170.005], "XYStage": [ 36583.7, -3787.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide20R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -3455.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -3455.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 38161.5, -2894.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide20R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19325.7], "PIEZO": [170.035], "XYStage": [ 37600.5, -2894.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide20R_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5171.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5171.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 561, 5732.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [170.01], "XYStage": [ 0, 5732.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 3849.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 3849.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 3849.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4410.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4410.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4410.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -2295.7, 4971.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1734.7, 4971.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19536.8], "PIEZO": [169.955], "XYStage": [ -1173.7, 4971.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3229 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3229 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ 323.6, 3790 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19547.5], "PIEZO": [170.01], "XYStage": [ -237.4, 3790 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 3665.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 3665.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 3665.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4226.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4226.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4226.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 4609.5, 4787.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30L_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5170.5, 4787.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30L_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19540.4], "PIEZO": [169.995], "XYStage": [ 5731.5, 4787.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30L_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -2358.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -2358.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -2358.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1797.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1797.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1797.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 4581.1, -1236.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5142.1, -1236.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19493.3], "PIEZO": [170.055], "XYStage": [ 5703.1, -1236.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -2505.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -2505.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ 162, -1944.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19512.8], "PIEZO": [169.99], "XYStage": [ -399, -1944.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -2071 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -2071 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -2071 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -1510 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -1510 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -1510 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -2545.1, -949 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1984.1, -949 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19502.9], "PIEZO": [169.985], "XYStage": [ -1423.1, -949 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq05_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, -549.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, -549.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ 491.5, 11.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq05_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19523.5], "PIEZO": [170.04], "XYStage": [ -69.5, 11.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq05_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "3aa3e31f-19e1-45d2-9307-cbe8f442a367", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 19:08:40 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 25, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq05_1", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 104, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 829188, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide20L_Seq05_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19355.3000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "47.2900", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 43811.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.06, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 19:09:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 4074.4, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }